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Dynamique et enzymologie de la digestion chez l'huitre Crassostrea gigas ArchiMer
Boucaud, C; Lebesnerais, C; Lubet, P; Lihrmann, I.
Feeding experiments carried out on Crassostrea gigas allowed the authors to set up the stages and the timing of the movement of food throughout the digestive tract. Various activities involved in digestion (glycosidases, esterases, and peptidases) were investigated histochemically in the digestive tract in order to localize the sites of absorption and the sites of protein and carbohydrate digestion. An explicative model of the different stages of digestion is proposed.
Tipo: Text Palavras-chave: Crassostrea gigas; Bivalvia; Cytology; Histochemistry; Histology; Digestive system; Enzymatic activity; Digestive glands; Digestion; Enzymes; Transit digestif; Digestion; Huitre; Crassostrea gigas.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1179.pdf
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Trophic relationships gonad-intestinal loop in Pecten maximus . ArchiMer
Lepennec, M; Dorange, G; Beninger, P; Donval, A; Widowati, I.
Histological, histochemical and ultrastructural studies were performed on the region of the gonad traversed by the intestine in Pecten maximus . The results indicate a digestive and absorptive function of intestine epithelial cells and a transfer of metabolites to the surrounding gonad tissue. Several possible mechanisms of translocation are presented, including diffusion into the haemolymph and transfer via vacuolated cells of the gonad interstitial connective tissue.
Tipo: Text Palavras-chave: Pecten maximus; Bivalvia; Histology; Haemolymph; Histochemistry; Cells; Metabolites; Epithelia; Intestines; Ultrastructure; Animal reproductive organs.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-1668.pdf
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Localisation immunohisto chimique de peptides apparentes aux hormones de vertebres chez Pecten maximus ArchiMer
Donval, A.
Substances related to vertebrate peptidic hormones have been searched by immunohistochemical methods in Pecten maximus . Neurosecretory cells have been detected in the nervous ganglia with antisera raised against vertebrate hormones, gastrin/CCK like, glucagon like, insuline like, somatostatine like. Other gastro-intestinal hormones like are localized in some cells of the gut epithelium: bombesine and vasoactive intestinal peptide.
Tipo: Text Palavras-chave: Pecten maximus; Bivalvia; Immunology; Histochemistry; Cells; Neurosecretory system; Juveniles; Hormones.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-1724.pdf
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Morphometry and acetylcholinesterase activity of the myenteric plexus of the wild mouse Calomys callosus BJMBR
The myenteric plexus of the digestive tract of the wild mouse Calomys callosus was examined using a histochemical method that selectively stains nerve cells, and the acetylcholinesterase (AChE) histochemical technique in whole-mount preparations. Neuronal density was 1,500 ± 116 neurons/cm2 (mean ± SEM) in the esophagus, 8,900 ± 1,518 in the stomach, 9,000 ± 711 in the jejunum and 13,100 ± 2,089 in the colon. The difference in neuronal density between the esophagus and other regions was statistically significant. The neuron profile area ranged from 45 to 1,100 µm2. The difference in nerve cell size between the jejunum and other regions was statistically significant. AChE-positive nerve fibers were distributed within the myenteric plexus which is formed by...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Myenteric plexus; Wild mouse; Calomys callosus; Morphometry; Histochemistry.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500009
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Morphological and histochemical analysis of the antennal gland of Macrobrachium Potiuna (Muller, 1880) (Crustácea, Decapoda, Palaemonidae) BABT
Moraes,Neci; Scaff,Rejane Cirra.
The objective of our study was to describe by histologic methods the structural organization of the antennal gland of M. potiuna as well as the location through histochemical methods of the neutral and sulfated polissacharides that exist in these regions. This study showed that the gland is composed of the a non-secretive tubular portion and a secretive portion which is formed by a cubic simple epithelium, sub-epithelium layer and cellular chains
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antennal gland; Macrobrachium potiuna; Morphology; Histochemistry.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300005
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Studies on the intrinsic nervous system of the wild rodent Calomys callosus digestive tract. II: The submucous plexus BJMBR
Souza,N.B.; Liberti,E.A.; De-Souza,R.R..
The submucous plexus of the normal small and large intestine of Calomys callosus was studied by NADH and AChE histochemical techniques and by transmission and scanning electron microscopy. The plexus contains (mean ± SD) 7,488 ± 293 neurons/cm2 in the duodenum, 5,611 ± 836 in the jejunum, 2,741 ± 360 in the ileum, 3,067 ± 179 in the cecum, and 3,817 ± 256 in the proximal colon. No ganglia or nerve cell bodies were seen in the esophagus, stomach, distal colon or rectum. The neurons are pear-shaped with a round or oval nucleus and the neuronal cell profile areas were larger in the large intestine than in the small intestine. Most of the neurons display intense AChE activity in the cytoplasm. AChE-positive nerve fibers are present in a primary meshwork of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Submucous plexus; Wild mouse; Morphometry; Histochemistry; Ultrastructure; Calomys callosus.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500007
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Development and some histochemical aspects of foliar glandular trichomes of Stevia rebaudiana (Bert.) Bert. - Asteraceae Rev. Bras. Bot.
MONTEIRO,WALKYRIA ROSSI; CASTRO,MARÍLIA DE MORAES; MAZZONI-VIVEIROS,SOLANGE CRISTINA; MAHLBERG,PAUL GORDON.
The ten-celled biseriate glandular trichome of Stevia rebaudiana (Bert.) Bert.-Asteraceae, found on both leaf surfaces, originates from a single protruding, protodermal cell undergoing an anticlinal division. A subsequent series of periclinal divisions, occurring in acropetal sequence, leads to the formation of the trichome, composed of five pairs of cells, one pair of basal cells, another of stalk cells and three pairs of secretory head cells. Developing, still two-celled glandular trichomes already occur on leaf primordia of the second pair (these primordia measuring, in some cases, ca. 0.30 mm in length), and most of the glandular trichomes are at the mature phase on very young, expanding leaves, for example on those of the sixth pair. The secretory...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stevia rebaudiana; Asteraceae; Glandular trichomes; Ontogeny; Histochemistry.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000300013
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Aspectos histológicos e histoquímicos da cloaca feminina de Columba livia (Gmelin) (Columbidae, Columbiformes) Zoologia
Teles,Maria Eloíza de Oliveira; Ribeiro,Maria das Graças; Maruch,Sandra Maria das Graças; Ribeiro,Roseli Deolinda.
The domestic dove Columba livia (Gmelin, 1789) is a species well adapted to Brazil and the study of its reproductive biology is part of a broad research project on birds. This essay describes the morphological aspects of the cloaca of female Columba livia, describing compartments lengthwise starting from the head such as the coprodeo, urodeo and proctodeo limited by mucosal folds. Each compartment of the cloaca presents its own morphological characteristics which differ one from the other in form, height and position of mucosal projections, kinds of tissues, presence and histological aspects of glands, presence of lymphonodus. The rectum opens into the coprodeo, the ureter opens into the oviduct and the cloacal sac opening into the proctodeo. Histochemical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Domestic dove; Morphological aspects; Histochemistry; Cloaca.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000100013
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Aspectos reprodutivos de Sphoeroides greeleyi (Gilbert), Pisces, Osteichthyes, Tetraodontidae, da gamboa do Baguaçu, Baia De Paranaguá, Paraná, Brasil Zoologia
Schultz,Yart Damasceno; Favaro,Luís Fernando; Spach,Henry Louis.
The present study analyses the reproductive aspects of Sphoeroides greeleyi (Gilbert, 1900), such as morphology and histochemistry of carbohydrates in the oogenesis phases, maturity scale, time and type of spawn, sexual rate and first ripened mature size for females. From the monthly captured animais (Nov 1998 to Nov 1999) the morphometric data (entire length and total weight) were extracted and the sex and the gonadal maturity were determined. Some ovaries were prepared for microscopic analysis that charac-terized six phases for the development of the ovarian folheies. Through the carbohydrates analysis the acid carbohydrates could be related to the oogenesis maintenance and the neutral carbohydrates to the beginning of embryonic development. A maturity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Reproduetion; Histology; Histochemistry.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752002000100004
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Ultramorphological analysis of the venom glands and their histochemical relationship with the convoluted glands in the primitive social paper wasp Polistes versicolor (Hymenoptera: Vespidae) J. Venom. Anim. Toxins incl. Trop. Dis.
Britto,F. B.; Caetano,F. H..
The venom glands are part of the most important defense weapon in Aculeata: the venom apparatus. The arrangement of these glands can vary among species, but in general they are composed of long secretory tubules connected to a muscular sac-like reservoir. Although the occurrence of these variations has been documented, many studies neglected the existence of a well-developed secretory portion in the lumen of the reservoir named convoluted gland. This study is an ultramorphological analysis of the venom glands and their histochemical relationship with the convoluted glands in the primitive social wasp Polistes versicolor. In this wasp, the venom glands are constituted by two tubular portions that penetrate individually in the venom reservoir, inside of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wasps; Venom glands; Convoluted glands; Ultramorphology; Histochemistry.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200007
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Effect of salbutamol on innervated and denervated rat soleus muscle BJMBR
Šoic-Vranic,T.; Bobinac,D.; Bajek,S.; Jerkovic,R.; Malnar-Dragojevic,D.; Nikolic,M..
The objective of the present investigation was to perform a 14-day time-course study of treatment with salbutamol, a ß2 adrenoceptor agonist, on rat soleus muscle in order to assess fiber type selectivity in the hypertrophic response and fiber type composition. Male Wistar rats were divided into four groups: control (N = 10), treated with salbutamol (N = 30), denervated (N = 30), and treated with salbutamol after denervation (N = 30). Salbutamol was injected intraperitoneally in the rats of the 2nd and 4th groups at a concentration of 0.3 mg/kg twice a day for 2 weeks. The muscles were denervated using the crush method with pean. The animals were sacrificed 3, 6, 9, 12, and 14 days after treatment. Frozen cross-sections of soleus muscle were stained for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salbutamol; Rat skeletal muscle; Soleus muscle; Denervation; Histochemistry; Muscle fibers.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200008
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Histochemical Features of the Extracellular Matrix in Rattus rattus norvegicus Otic Ganglion International Journal of Morphology
Chagas,M. A.; Babinski,M. A.; Abidu-Figueiredo,M.; Costa,W. S..
The otic ganglion is a cranial component of the parasympathetic division of the autonomic nervous system (ANS). Similar to other parasympathetic ganglia, otic ganglion presents multipolar neurons that are completely surrounded by satellite cells and intercellular substance as well, which allow us to use this ganglion as a good experimental model for studying the relationship neuron / extracellular matrix. We have studied rat otic ganglion in 10 animals through light microscopy. After routine histological methods, 5 µm sections were obtained and coloured by Gomori`s trichrome, periodic acid-Schiff (PAS), Alcian Blue pH 2.5 and pH1.0, acetylation + PAS, acetylation + deacetylation + PAS, acid hydrolysis + PAS, phenylhydrazine + PAS and thiosemicarbazide +...
Tipo: Journal article Palavras-chave: Rat; Otic ganglion; Autonomic nervous system; Histochemistry.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000100015
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Mucous Membrane of the Rumen of Ovines, Fed With Spineless, Forrage Cactus or Palm (Barbary Fig) (Opuntia ficus indica Mil): Hystochemical Study by Means of Light Microscopy International Journal of Morphology
Neiva,Gentileza Santos Martins; da Mota,Diógenes Luís; Batista,Ângela Maria Vieira; Sousa-Rodrigues,Célio Fernando de.
The mucosa of the rumen performs the functions of absorption, transportation, metabolism and protection, due to the presence of microorganisms in this compartment of the digestive tract, which are responsible for the digestion of approximately 60% of the organic material apparently digested. The histological structure and the histochemical profile of glicoconjugate of the mucosa of the stomach of sheep that were being fed with forrage cactus or palm (Giant Palm - Opuntia ficus indica Mil)1. Twelve sheep were used, with approximately 14 months old, which were oddly distributed in four diets: D1- 60.0% forrage palm + 40.0% concentrated ration; D2- 47.5% forrage palm + 12.5% elephant, napier grass + 40.0% concentrated ration, and D3 - 34,8% forrage palm +...
Tipo: Journal article Palavras-chave: Mucous membrane; Histology; Histochemistry; Forrage palm; Forrage cactus; Ovine.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500034
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Morphometry and Histochemistry of the Rectus abdominis Muscle Fibers of Tufted Capuchin Monkeys (Cebus apella Linnaeus, 1758) International Journal of Morphology
Simionato,L. H.; Andreo,J. C.; Oliveira,J. A. de; Bortoluci,C. H. F.; dos Santos,N. B.; Moraes,L. H. R.; Rodrigues,A. C.; Andreo,M. B..
The phylogenetic proximity of primates to humans, along with their behavioral, biochemical, and anatomical similarities, make such animals more interesting experimental models for biomedical researches, as compared to classical laboratory animals. Another aspect that has called the attention of researchers is the differentiated quadrupedalism present in some primates. The tufted capuchin monkey uses the ground and tree branches as its support for locomotion, showing various postures while performing this task. On the basis of this information, we have decided to study the rectus abdominis muscle of the tufted capuchin monkey, with the following goals: the frequency and area of fiber types; its possible compartmentalization; and identify if this muscle is...
Tipo: Journal article Palavras-chave: Rectus abdominis muscle; Histochemistry; Musculars fiber types; Tufted capuchin monkey; Cebus apella; Primates; Simians.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000100011
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Structural, hystochemical and cytochemical characteristics of the stigma and style in Passiflora edulis f. flavicarpa (Passifloraceae) BABT
Souza,Margarete Magalhães; Pereira,Telma Nair Santana; Dias,Angelo José Burla; Ribeiro,Beatriz Ferreira; Viana,Alexandre Pio.
Studies were carried out on anatomic aspects of the stigma and style of yellow passionfruit. The stigmatic style consisted of papillae, many layers of sub-epidermal cells and a central transmitting tissue. The stigma was of dry, unbranched, multicellular multiseriate papillate type and the cells were highly vacuolated with walls that were relatively thin consisting of compactly arranged fibrils. The style was solid with a central core of transmitting tissue that had intercellular spaces containing a matrix that included polysaccharidic and lipidic substances. During development, cells of the transmitting tissue were arranged compactly while the mature pistil showed cells with a little more loosely arranged intercellular spaces. Both the mature stigma and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yellow passionfruit; Anatomy; Stigma and style; Histochemistry; Cytochemistry.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100011
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Clinical and Histochemical Alterations of the Periodontal Ligament in Gerbils after Malocclusion Induced International Journal of Morphology
Naves,Leandro Moura Leite; Issa,João Paulo Mardegan; Pitol,Dimitrius Leonardo; Fukada,Sandra Yasuyo; di Matteo,Miguel Ángel Sala; Iyomasa,Mamie Mizusaki.
The aim of this article is to show the clinical and histochemical alterations of the first periodontal ligament, on the right side, after upper molars teeth extraction on the left side in gerbils. After two months, the periodontal ligaments were removed and processed for histochemical analysis. The data showed that TRAP reaction was able to evidence the osteoclastic activity in the hyperfunction hemimandible, right side, explaining the functional changes in the periodontal ligament after teeth extraction, and a little gingival recession and radicular exposure of teeth without function was observed at inferior molars of the left side
Tipo: Journal article Palavras-chave: Periodontal ligament; Histochemistry; Malocclusion; Gerbil; Meriones unguiculatus.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000400036
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Androgen Receptor Distribution, PAS and Alcyan Blue Reaction in the Vomeronasal Organ and the Nasal Septum Mucosa of the Developing Male Rat International Journal of Morphology
Núñez Chichet,M E; Genovese,P; Bielli,A.
The vomeronasal organ (VNO) is a tubular chemoreceptory organ which detects environmental pheromones and is essential for mammalian normal reproductive activity. A sensitive chemoreceptory neuroepithelium lines the concave VNO wall and the opposite, convex wall is lined by a seudostratified, receptor-free epithelium. The secretion of the Jacobson glands (JG) -tubuloacinary glands lying in the lamina propia of the VNO - is essential for pheromones contacting vomeronasal organ chemoreceptors. Tubuloacinary glands lying in the connective tissue of the nasal septum mucosa (Bowman glands), can be classified as ventral and dorsal, according to their location. Positivity to PAS and Alcyan blue reactions and androgen receptor immunolocalization was evaluated with...
Tipo: Journal article Palavras-chave: Vomeronasal organ; Chemoreception; Development; Histochemistry; Immunohistochemistry.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300018
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Morphological and histochemical study of the masseter muscle after occlusal alteration Biocell
Mardegan Issa,João Paulo; Tiossi,Rodrigo; Mizusaki Iyomasa,Mamie.
The aim of this study was to evaluate alterations in the masseter muscle of 30 male guinea-pigs submitted to occlusal alteration. The animals were divided into 2 equal groups, the control group (C) only submitted to surgical stress, and the occlusal altered group (T) submitted to teeth extraction. Each group was subdivided into 3 groups, with 5 animals, for the following studies: macroscopy and vessels distribution, light microscopy and histochemical analysis, with animals' perfusion 2 months after surgery. Data were submitted to statistical analysis. Macroscopically, an anteroposterior orientation of the muscular fibers was found and statistical difference between C and T groups in width (p<0.05). Microscopically, C and T groups showed polygonal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Masseter muscle; Morphology; Histology; Histochemistry.
Ano: 2007 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300003
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Comparative Intestinal Histomorphology of Five Species of Phyllostomid Bats (Phyllostomidae, Microchiroptera): Ecomorphological Relations with Alimentary Habits International Journal of Morphology
Gadelha-Alves,Rafaela; Rozensztranch,Andréa Melo Da Silva; Rocha- Barbosa,Oscar.
The intestines of Sturnira lilium, Phyllostomus hastatus, Carolliaperspicillata, GlossophagasoricinaíindDesmodus rotundus were analized through macroscopic, morphometric and light microscopy studies. The species studied presented some common charactenstics generally found in bats, such as the absence of a caecum and an appendix. The frugivorous bat S. lilium has a longer intestine than the other species. The intestinal epithelium is of the simple columnar type, constituted of absorptive cells characterized by microvilli and goblet cells, which in all the species proved to be PAS + and AB +. These cells presented some differences in quantity and in distribution among the species. The intestinal mucosa presented morphologic and morphometric differences, such...
Tipo: Journal article Palavras-chave: Intestines; Bats; Phyllostomidae; Histology; Histochemistry; Micrometry; Ecomorphology.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000300014
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Histology and histochemistry of the venom apparatus of the black scorpion Androctonus crassicauda (Olivier, 1807) (Scorpiones: Buthidae) J. Venom. Anim. Toxins incl. Trop. Dis.
Jarrar,BM; Al-Rowaily,MA.
The venom apparatus of the black scorpion Androctonus crassicauda has been characterized histologically and histochemically in the present study. The results showed that this apparatus consists of paired venom glands, each of which initially presents its own canal and posteriorily both fuse into a single common one. Each gland is covered by a sheath of striated muscle and is lined with extensively folded secretory epithelium (formed of non-secretory and secretory venom-producing cells). The outcomes also revealed that the venom-producing cells of both glands produce neutral mucosubstances, sialomucins, sulfomucins and proteins, but are devoid of glycogen. Cysteine, tyrosine, tryptophan and arginine were also detected along with activities of acid and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Androctonus; Scorpion; Venom apparatus; Histochemistry; Phosphatases; Esterases; Peptidases.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300010
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